Magnetization Transfer Detection of GFP: A New MRI Gene Reporter
نویسندگان
چکیده
for GFP vs. BSA comparison. B. Region based MTR calculations for the different frequency offsets for GFP vs. BSA Protein comparison. P-values <0.01 for 0.5, 1, and 5 kHz C. Pseudocolored pixel by pixel MTR calculation for 1 kHz offset for Actin GFP model. D. Region based MTR calculations for the different frequency offsets for Actin GFP model. P-values <0.05 for 10 kHz, <0.01 for 1 and 5 kHz, and <0.001 for 0.5. A. B.
منابع مشابه
A New Reporter Gene Technology: Opportunities and Perspectives
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
متن کاملIn vitro and in vivo magnetic resonance imaging (MRI) detection of GFP through magnetization transfer contrast (MTC)
Green fluorescent protein (GFP) is a widely utilized molecular marker of gene expression. However, its use in in vivo imaging has been restricted to transparent tissue mainly due to the tissue penetrance limitation of optical imaging. Here, we report a novel approach to detect GFP with Magnetization transfer contrast (MTC) magnetic resonance imaging (MRI). MTC is an MRI methodology currently ut...
متن کاملSynthesis a New Viral Base Vector Carrying Single Guide RNA (sgRNA) and Green Florescent Protein (GFP)
CRISPR/Cas9 system is a powerful gene editing tool in vivo and in vitro. Currently, CRISPR/Cas9 delivery cells or tissue with different vehicles are available, and Adeno- associated virus (AAV) in one of them. Due to AAV packaging size limitation, AAV base vectors that carry CRISPR/Cas9 system do not have florescent tag like GFP for simple detection and navigation of cells, containing AAV. The ...
متن کاملInvestigation of an Optimized Context for the Expression of GFP as a Reporter Gene in Chlamydomonas Reinhardtii
Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...
متن کاملIn vivo detection of cFos-GFP for neural activation via Magnetization Transfer Contrast (MTC) Magnetic Resonance Imaging (MRI)
overlay (short axis view) from a cFos-GFP mouse exposed to a dehydration protocol normalized to control. We collected 3D MTC datasets before the dehydration protocol and also 2 hours after dehydration for one control and one cFos-GFP mouse. The bright areas correspond with increased MTR in cFos-GFP mice in comparison to control. In this case, we observed an increase in the hypothalamic PVN (red...
متن کامل